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Addgene

pcDNA5/FRT/TO-TRPT1
(Plasmid #184244)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184244 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Total vector size (bp) 5961
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRPT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    762
  • Entrez Gene
    TRPT1
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO-TRPT1 was a gift from Karla Feijs (Addgene plasmid # 184244 ; http://n2t.net/addgene:184244 ; RRID:Addgene_184244)

  • For your References section:

    ADP-ribosylation of RNA in mammalian cells is mediated by TRPT1 and multiple PARPs. Weixler L, Feijs KLH, Zaja R. Nucleic Acids Res. 2022 Sep 9;50(16):9426-9441. doi: 10.1093/nar/gkac711. 10.1093/nar/gkac711 PubMed 36018800
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