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Addgene

LV-Camk2-ChroMEd15-mTFP
(Plasmid #184240)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184240 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LV-Camk2
  • Backbone size w/o insert (bp) 9239
  • Total vector size (bp) 10976
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChroMEd15-mTFP
  • Insert Size (bp)
    1737
  • Mutation
    deleted ChroME amino acids 317-331
  • Promoter Camk2
  • Tag / Fusion Protein
    • mTFP1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GTCAAGCCGGTTCTCCGTTTGCACTC
  • 3′ sequencing primer CAGCGTATCCACATAGCGTAAAAGGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV-Camk2-ChroMEd15-mTFP was a gift from Yiyang Gong (Addgene plasmid # 184240 ; http://n2t.net/addgene:184240 ; RRID:Addgene_184240)

  • For your References section:

    Engineering rhodopsins' activation spectra using a FRET-based approach. Beck C, Gong Y. Biophys J. 2022 Mar 21. pii: S0006-3495(22)00234-X. doi: 10.1016/j.bpj.2022.03.024. 10.1016/j.bpj.2022.03.024 PubMed 35331688
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