pDEST17-hTRPT1-iso1
(Plasmid
#184239)
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PurposeFor purification of N-terminally His-tagged TRPT1 from bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184239 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEST17
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5463
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRPT1
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SpeciesH. sapiens (human)
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Insert Size (bp)762
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Entrez GeneTRPT1
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7fw (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST17-hTRPT1-iso1 was a gift from Karla Feijs (Addgene plasmid # 184239 ; http://n2t.net/addgene:184239 ; RRID:Addgene_184239) -
For your References section:
ADP-ribosylation of RNA in mammalian cells is mediated by TRPT1 and multiple PARPs. Weixler L, Feijs KLH, Zaja R. Nucleic Acids Res. 2022 Sep 9;50(16):9426-9441. doi: 10.1093/nar/gkac711. 10.1093/nar/gkac711 PubMed 36018800