AviTag-SpyTag003-MBP
(Plasmid
#184227)
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PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5219
- Total vector size (bp) 6515
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAviTag-SpyTag003-MBP
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SpeciesSynthetic
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Insert Size (bp)1296
- Promoter T7
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Tags
/ Fusion Proteins
- His6 (N terminal on insert)
- Thrombin cleavage site (N terminal on insert)
- AviTag (N terminal on insert)
- SpyTag003 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AviTag-SpyTag003-MBP was a gift from Mark Howarth (Addgene plasmid # 184227 ; http://n2t.net/addgene:184227 ; RRID:Addgene_184227) -
For your References section:
SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M. Nat Commun. 2022 Jun 28;13(1):3714. doi: 10.1038/s41467-022-31193-8. 10.1038/s41467-022-31193-8 PubMed 35764623
