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Addgene

SpyTag-sfGFP
(Plasmid #184226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ404
  • Backbone size w/o insert (bp) 3987
  • Total vector size (bp) 4821
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyTag-sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    834
  • Promoter T5
  • Tags / Fusion Proteins
    • SpyTag (N terminal on insert)
    • TEV protease cleavage site (N terminal on insert)
    • His6 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCGCTCACAATTCCACAACGGTTTCCC
  • 3′ sequencing primer CGAAAGGCTCAGTCGAAAGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpyTag-sfGFP was a gift from Mark Howarth (Addgene plasmid # 184226 ; http://n2t.net/addgene:184226 ; RRID:Addgene_184226)

  • For your References section:

    SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M. Nat Commun. 2022 Jun 28;13(1):3714. doi: 10.1038/s41467-022-31193-8. 10.1038/s41467-022-31193-8 PubMed 35764623
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