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Addgene

Anti-GluA2 [L21/32R]
(Antibody #184172)

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Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 184172-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 184172-rAb.T 20 µg of purified recombinant antibody $85 *

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Features

Production & Usage

  • Production Plasmid
    Anti-GluA2 [L21/32R]
  • Antigen Plasmid
    GluR2 FL/pRK5
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20°C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2750683

Terms and Licenses

Target Antigen

Protein Glutamate receptor 2
Antigen Description Amino acids 834–883 cytoplasmic C-terminus of rat GluA2/GluR2
Antigen Amino Acid Sequence
EFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI
Species R. norvegicus (rat)
Additional Species Reactivity H. sapiens (human),  M. musculus (mouse)
Gene Gria2
Alternative Names
  • AMPA-selective glutamate receptor 2
  • GluR-B
  • GluR-K2
  • Glutamate receptor ionotropic
  • AMPA 2
  • GluA2
External References

Applications (2)

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Immunohistochemistry

1 image
Immunohistochemistry image by James Trimmer, UC Davis
Submitted By: James Trimmer, UC Davis
Addgene Partner
Antibody Type
Recombinant
Sample Species
R. norvegicus (rat)
Result
Pass
1 image
Immunohistochemistry image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass

Depositor Comments

Does not cross-react with GluA1/GluR1, GluA3/GluR3 or GluA4/GluR4 (based on KO validation results).
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-GluA2 [L21/32R] - from James Trimmer (Addgene antibody # 184172 ; http://n2t.net/addgene:184172 ; RRID:AB_2750683)
  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360