Anti-LRRK2/Dardarin, C-terminus [N241A/34R]
(Antibody
#184170)
-
PurposeAnti-LRRK2/Dardarin, C-terminus (Human) recombinant mouse monoclonal antibody
-
Depositing Lab
-
Recommended Applications
Image 1 (of 3): Western Blot
Crude rat membranes immunoblotted against Anti-LRRK2/Dardarin, C-terminus hybridoma-deriv…
View details
Credit: James Trimmer, UC Davis
Image 2 (of 3): Immunohistochemistry
Delipidated mouse brain tissue was stained with Anti-LRRK2/Dardarin, C-terminus [N241A/34…
View details
Credit: Zhuhao Wu, Weill Cornell Medicine
Image 3 (of 3): Immunocytochemistry
COS cells were transfected with an antigen plasmid expressing Myc-tagged LRRK2 and staine…
View details
Credit: James Trimmer, UC Davis
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Recombinant Antibody | 184170-rAb | 100 µg of purified recombinant antibody | $250 * | ||
| Recombinant Antibody trial size | 184170-rAb.T | 20 µg of purified recombinant antibody | $85 * | ||
* Log in to view industry pricing.
Features
-
Source SpeciesM. musculus (mouse)
-
IsotypeIgG2a
-
Light Chain Typekappa
-
Amino Acid Sequences
Sequences (2) — Accept Affinity Reagent Sequence Policy
Production & Usage
-
Production PlasmidAnti-LRRK2/Dardarin, C-terminus [N241A/34R]
-
Antigen PlasmidLRRK2-WT
-
PurificationPurified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
-
Storage BufferPBS + 1 mM sodium azide
Delivery
-
Concentration0.9–1.1 mg/mL
-
StorageUpon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
-
ShipmentBlue ice or room temp
Resource Information
-
Persistent Resource IdentifierRRID:AB_2909565
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Zeocin® is an InvivoGen trademark.
Trademarks:
Target Antigen
| Protein | Leucine-rich repeat serine/threonine-protein kinase 2 |
|---|---|
| Antigen Description | Amino acids 970–2527 (C-terminus) of human LRRK2 |
| Antigen Amino Acid Sequence |
|
| Species | H. sapiens (human) |
| Additional Species Reactivity | M. musculus (mouse), R. norvegicus (rat) |
| Gene | LRRK2 |
| Alternative Names |
|
| External References |
|
Applications (6)
Immunocytochemistry
Immunohistochemistry
Western Blot
Knockout validation
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Anti-LRRK2/Dardarin, C-terminus [N241A/34R] - from James Trimmer (Addgene antibody # 184170 ; http://n2t.net/addgene:184170 ; RRID:AB_2909565) -
For your References section:
A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360