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pMC_mNG2(11)_BSDminus_F2
(Plasmid #184164)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184164 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMC.BESPX
  • Vector type
    CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    mNG2(11)
  • Alt name
    mNeonGreen2(11)
  • Species
    Branchiostoma lanceolatum

Gene/Insert 2

  • Gene/Insert name
    bsd
  • Alt name
    Blasticidin-S deaminase
  • Species
    Aspergillus terreus

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMC_mNG2(11)_BSDminus_F2 was a gift from Ophir Shalem (Addgene plasmid # 184164 ; http://n2t.net/addgene:184164 ; RRID:Addgene_184164)

  • For your References section:

    Efficient and flexible tagging of endogenous genes by homology-independent intron targeting. Serebrenik YV, Sansbury SE, Kumar SS, Henao-Mejia J, Shalem O. Genome Res. 2019 Aug;29(8):1322-1328. doi: 10.1101/gr.246413.118. Epub 2019 Jun 25. 10.1101/gr.246413.118 PubMed 31239279
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