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Addgene

pLY232
(Plasmid #184149)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184149 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSEVA221
  • Backbone manufacturer
    by de Lorenzo's Lab (The Standard European Vector Architecture (SEVA) platform)
  • Backbone size w/o insert (bp) 3821
  • Total vector size (bp) 5264
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Culture in the Lennox’s Luria-Bertani (LB) medium (10 g/L peptone, 5 g/L yeast extract, 5 g/L NaCl)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    s-tracrRNA-WT
  • Species
    Synthetic
  • Insert Size (bp)
    1466
  • Promoter araBAD promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer AGGGCGGCGGATTTGTCC
  • 3′ sequencing primer GCGGCAACCGAGCGTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLY232 was a gift from Baojun Wang (Addgene plasmid # 184149 ; http://n2t.net/addgene:184149 ; RRID:Addgene_184149)

  • For your References section:

    Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and sequence-specific RNA biosensors. Liu Y, Pinto F, Wan X, Yang Z, Peng S, Li M, Cooper JM, Xie Z, French CE, Wang B. Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x. 10.1038/s41467-022-29604-x PubMed 35410423
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