pLY185
(Plasmid #184146)

• Purpose: RFP generator controlled by Plux2 promoter
• Depositing Lab: Baojun Wang
• Publication: Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi: 10.1038/s41467-022-29604-x.

Backbone

• Vector backbone: p15AC
• Backbone manufacturer: p15A vector from pdCas9-bacteria (Plasmid #44249)
• Backbone size w/o insert (bp): 1725
• Total vector size (bp): 3593
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Culture in the Lennox’s Luria-Bertani (LB) medium (10 g/L peptone, 5 g/L yeast extract, 5 g/L NaCl)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: rfp
• Species: Synthetic
• Insert Size (bp): 1847
• Promoter: Plux2

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: SpeI (destroyed during cloning)
• 5′ sequencing primer: AACGGTCTGGTTATAGGTACATTGAGCAAC
• 3′ sequencing primer: GTTCGTAAGCCATTTCCGCTCGCCGCAGTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry