pJL6
(Plasmid #184134)

• Purpose: For labelling (RFP) the apical region of the intestine. Translational fusion construct. coel::RFP_Pvha-6::vha-6::dsred.
• Depositing Lab: Alejandro Aballay
• Publication: Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 10.1038/s42003-022-03381-1.

Backbone

• Vector backbone: coel::rfp
• Backbone manufacturer: Piali Sengupta Lab
• Backbone size w/o insert (bp): 4194
• Total vector size (bp): 8243
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CELE_VW02B12L.1
• Alt name: vha-6
• Species: C. elegans (nematode)
• Entrez Gene: vha-6 (a.k.a. CELE_VW02B12L.1)
• Promoter: Pvha-6 (894bp)
• Tag / Fusion Protein:
  • RFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SphI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: M13 Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry