pJL3
(Plasmid #184131)

• Purpose: For rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.
• Depositing Lab: Alejandro Aballay
• Publication: Lalsiamthara et al Commun Biol. 2022 May 5;5(1):422. doi: 10.1038/s42003-022-03381-1.

Backbone

• Vector backbone: pPD95.75
• Backbone manufacturer: Fire Lab
• Backbone size w/o insert (bp): 4376
• Total vector size (bp): 12293
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MRP-1
• Alt name: MRP1
• Alt name: ABCC1
• Species: C. elegans (nematode)
• Insert Size (bp): 7668
• Entrez Gene: mrp-1 (a.k.a. CELE_F57C12.5)
• Promoter: Pges-1 (3.3kb)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SphI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: M13 Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry