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pT2iApowriter2(#336)
(Plasmid #184104)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184104 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT2i (pT2AL200R150G derivative)
  • Backbone manufacturer
    modified by M Volovitch from pT2AL200R150 of A Urasaki; Urasaki et al 2006, Genetics 174:639)
  • Backbone size w/o insert (bp) 6190
  • Vector type
    Zebrafish transient transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
  • Species
    Rous Sarcoma V (myr), fruitfly (DIAP1), human (myc, ERT2), rodent Hepacivirus (nls'), PTV-1 (P2A), mouse (Caspase9)
  • Insert Size (bp)
    3875
  • Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter Zf-Ubi
  • Tag / Fusion Protein
    • internal tag: 5myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SnaBI (not destroyed)
  • 5′ sequencing primer ubidir
  • 3′ sequencing primer SVLrev34
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic ERT2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

myr; DIAP1: NP_001261916; 5myc; tEosFP: AAV54099; nls; P2A: AIE57569; Casp9: NP_056548; ERT2: NP_000116; polycistronic mRNA expressing both inducible apoptosis trigger (Casp9-ERT2) and apoptosis sensor (myr-DIAP1-tEosFP-nls);

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2iApowriter2(#336) was a gift from Sophie Vriz (Addgene plasmid # 184104 ; http://n2t.net/addgene:184104 ; RRID:Addgene_184104)

  • For your References section:

    Optical control and study of biological processes at the single-cell level in a live organism. Feng Z, Zhang W, Xu J, Gauron C, Ducos B, Vriz S, Volovitch M, Jullien L, Weiss S, Bensimon D. Rep Prog Phys. 2013 Jul;76(7):072601. doi: 10.1088/0034-4885/76/7/072601. Epub 2013 Jun 14. 10.1088/0034-4885/76/7/072601 PubMed 23764902
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