GST-SmB
(Plasmid
#1841)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2TK
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmB
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Alt namesplicing factor
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Alt namesnRNP protein
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Alt nameSNRPB
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SpeciesH. sapiens (human)
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Insert Size (bp)250
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Entrez GeneSNRPB (a.k.a. CCMS, COD, SNRPB1, Sm-B/B', SmB/B', SmB/SmB', snRNP-B)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
(Leder #C-1091)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-SmB was a gift from Philip Leder (Addgene plasmid # 1841 ; http://n2t.net/addgene:1841 ; RRID:Addgene_1841) -
For your References section:
WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif. Bedford MT, Reed R, Leder P. Proc Natl Acad Sci U S A 1998 Sep 1;95(18):10602-7. 10.1073/pnas.95.18.10602 PubMed 9724750