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pCSmEn25EERT(#232)
(Plasmid #184081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone manufacturer
    DL Turner (Turner & Weintraub, 1994, G&Dev 8:1434)
  • Backbone size w/o insert (bp) 4058
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    6his-myc-chkEn2(5E)-ERT2
  • Species
    Synthetic; (6his), H. sapiens (myc), G. gallus (chkEn2), H. sapiens (ERT2)
  • Insert Size (bp)
    2009
  • Mutation
    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter sCMV+SP6
  • Tag / Fusion Protein
    • 6his-Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SVLrev34
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ERT2 (Feil and al 1997 BBRC 237:752) received from Daniel Metzger & Pierre Chambon in may 2004

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

6myc; chkEn2: NP_001254648; ERT2: NP_000116;

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSmEn25EERT(#232) was a gift from Sophie Vriz (Addgene plasmid # 184081 ; http://n2t.net/addgene:184081 ; RRID:Addgene_184081)

  • For your References section:

    Control of brain patterning by Engrailed paracrine transfer: a new function of the Pbx interaction domain. Rampon C, Gauron C, Lin T, Meda F, Dupont E, Cosson A, Ipendey E, Frerot A, Aujard I, Le Saux T, Bensimon D, Jullien L, Volovitch M, Vriz S, Joliot A. Development. 2015 May 15;142(10):1840-9. doi: 10.1242/dev.114181. Epub 2015 Apr 29. 10.1242/dev.114181 PubMed 25926358
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