pCSZPKIaHAERT(#118)
(Plasmid #184075)

• Purpose: cyclofen-inducible PK inhibitor alpha (wt) activation via mammalian cell transfection or mRNA synthesis
• Depositing Lab: Sophie Vriz
• Publication: Vriz lab cyclofen-inducible ERT2 plasmids (unpublished)

Backbone

• Vector backbone: pCS2
• Backbone manufacturer: DL Turner (Turner & Weintraub, 1994, G&Dev 8:1434)
• Backbone size w/o insert (bp): 4056
• Vector type: Mammalian Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZfPKAinhiba-HA-ERT2
• Species: D. rerio (ZfPKinhibalpha), Influenza A Virus (HA), H. sapiens (ERT2)
• Insert Size (bp): 1282
• Mutation: ERT2: G400V, M543A, L544A, deleted 1-281;
• Promoter: sCMV+SP6
• Tag / Fusion Protein:
  • internal tag: HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BsaBI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SVLrev34

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ERT2 (Feil and al 1997 BBRC 237:752) received from Daniel Metzger & Pierre Chambon in may 2004

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ZfPKIa: NP_001116417; HA; ERT2: NP_000116;