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pT2iaTubG4FER3(#940)
(Plasmid #184071)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184071 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT2i (pT2AL200R150G derivative)
  • Backbone manufacturer
    modified by M Volovitch from pT2AL200R150 of A Urasaki; Urasaki et al 2006, Genetics 174:639)
  • Backbone size w/o insert (bp) 3142
  • Vector type
    Zebrafish transient transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gal4bdFF-ERT2
  • Species
    S. cerevisiae (Gal4bd), Herpes Simplex Virus (F), H. sapiens (ERT2)
  • Insert Size (bp)
    1510
  • Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter Gfish-a1Tub

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BsaBI (not destroyed)
  • 5′ sequencing primer a1tubpr
  • 3′ sequencing primer SVLrev34
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic ERT2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gal4FF: AJD87368; ERT2: NP_000116;

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2iaTubG4FER3(#940) was a gift from Sophie Vriz (Addgene plasmid # 184071 ; http://n2t.net/addgene:184071 ; RRID:Addgene_184071)
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