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pT22UbGfpnE4PCh(#301)
(Plasmid #184066)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT22i (pT2AL200R150G derivative)
  • Backbone manufacturer
    modified by M Volovitch from pT2AL200R150 of A Urasaki; Urasaki et al 2006, Genetics 174:639)
  • Backbone size w/o insert (bp) 8046
  • Vector type
    Zebrafish transgenesis (blue eyes)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP-nls-ERT2-P2A-mCherry
  • Species
    Aequora victoria (GFP), SV40 (nls), H. sapiens (ERT2), PTV-1 (P2A), Discosoma sp. (mCherry)
  • Insert Size (bp)
    2519
  • Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter Zf-Ubi

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer ubidir
  • 3′ sequencing primer SVLrev34
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic ERT2

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

eGPF: AAB02572; nls; ERT2: NP_000116; P2A: AIE57569; mCherry: AY678264 (polycistronic mRNA: mCherry cytoplasmic marker of expression) + gCRY:CFP as a marker of transgenesis;

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT22UbGfpnE4PCh(#301) was a gift from Sophie Vriz (Addgene plasmid # 184066 ; http://n2t.net/addgene:184066 ; RRID:Addgene_184066)

  • For your References section:

    Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging. Zhang W, Hamouri F, Feng Z, Aujard I, Ducos B, Ye S, Weiss S, Volovitch M, Vriz S, Jullien L, Bensimon D. Chembiochem. 2018 Jun 18;19(12):1232-1238. doi: 10.1002/cbic.201700630. Epub 2018 Feb 27. 10.1002/cbic.201700630 PubMed 29341391
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