pT2h70CRET(#325)
(Plasmid
#184060)
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Purposecyclofen-inducible CRE activation in zebrafish transient transgenic
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSmart-LowCopy/pT2KXIGdin hybrid
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Backbone manufacturerLucigen Corp
- Backbone size w/o insert (bp) 5767
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Vector typeZebrafish transient transgenesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCRE-ERT2
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SpeciesPhage P1 (CRE), H. sapiens (ERT2)
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Insert Size (bp)2015
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MutationERT2: G400V, M543A, L544A, deleted 1-281;
- Promoter Zf-Hsp70-4
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site StuI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer SVErev2 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byERT2 (Feil and al 1997 BBRC 237:752) received from Daniel Metzger & Pierre Chambon in may 2004
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CRE: YP_006472; ERT2: NP_000116;
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2h70CRET(#325) was a gift from Sophie Vriz (Addgene plasmid # 184060 ; http://n2t.net/addgene:184060 ; RRID:Addgene_184060) -
For your References section:
Optical Control of Tumor Induction in the Zebrafish. Feng Z, Nam S, Hamouri F, Aujard I, Ducos B, Vriz S, Volovitch M, Jullien L, Lin S, Weiss S, Bensimon D. Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.1038/s41598-017-09697-x. 10.1038/s41598-017-09697-x PubMed 28835665