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pCSnERT(#186)
(Plasmid #184057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184057 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone manufacturer
    DL Turner (Turner & Weintraub, 1994, G&Dev 8:1434)
  • Backbone size w/o insert (bp) 4050
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nls-ERT2
  • Species
    SV40 (nls), H. sapiens (ERT2)
  • Insert Size (bp)
    1040
  • Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter sCMV+SP6
  • Tag / Fusion Protein
    • NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoO109I (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SVLrev34
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ERT2 (Feil and al 1997 BBRC 237:752) received from Daniel Metzger & Pierre Chambon in may 2004

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ERT2: NP_000116;

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSnERT(#186) was a gift from Sophie Vriz (Addgene plasmid # 184057 ; http://n2t.net/addgene:184057 ; RRID:Addgene_184057)
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