A1-20B1(P004)
(Plasmid
#184056)
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PurposeThis plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184056 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-Blunt
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3544
- Total vector size (bp) 4348
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameA1-20B1 preamplifier
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Alt nameA1 preamplifier
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SpeciesSynthetic
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Insert Size (bp)804
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 reverse
- 3′ sequencing primer M13 forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.10.20.465061v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
A1-20B1(P004) was a gift from C. Ron Yu (Addgene plasmid # 184056 ; http://n2t.net/addgene:184056 ; RRID:Addgene_184056) -
For your References section:
Robust and sensitive in situ RNA detection using Yn-situ. Wu Y, Xu W, Ma L, Yu Z, Wang Y, Yu CR. Cell Rep Methods. 2022 Apr 18;2(4):100201. doi: 10.1016/j.crmeth.2022.100201. eCollection 2022 Apr 25. 10.1016/j.crmeth.2022.100201 PubMed 35497500