pcDNA3-Lyso-INPP4B-mCherry
(Plasmid #184055)

• Purpose: Lysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.
• Depositing Lab: Jin Zhang
• Publication: Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-2020. doi: 10.1021/acscentsci.1c00919. Epub 2021 Dec 3.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5415
• Total vector size (bp): 10179
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LGP120 Lyso-INPP4B-mCherry
• Alt name: LAMP1-INPP4B-mCherry
• Species: H. sapiens (human)
• Insert Size (bp): 4764
• Entrez Gene: LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
• Entrez Gene: INPP4B
• Promoter: CMV
• Tags / Fusion Proteins:
  • Lysosome-associated membrane protein 1 (LAMP1). (N terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-Lyso-INPP4B-mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: INPP4B cDNA was provided by Gerry Hammond (University of Pittsburgh School of Medicine).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry