pcDNA3-Lyso-PTEN(A4)-mCherry
(Plasmid #184054)

• Purpose: Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.
• Depositing Lab: Jin Zhang
• Publication: Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-2020. doi: 10.1021/acscentsci.1c00919. Epub 2021 Dec 3.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5415
• Total vector size (bp): 8622
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lyso-PTEN(A4)-mCherry
• Alt name: LAMP1-PTEN(S380A,T382/383A,S385A)-mCherry
• Species: H. sapiens (human)
• Mutation: Ser 380/385 and Thr 382/383 in PTEN mutated to Ala.
• Entrez Gene: PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Lysosome-associated membrane protein 1 (LAMP1). (N terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-Lyso-PTEN(A4)-mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry