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Addgene

pcDNA3-Lyso-InPAkt
(Plasmid #184053)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184053 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5415
  • Total vector size (bp) 8667
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lyso-InPAkt
  • Alt name
    LAMP1-truncECFP-PH(Akt)-pseudoligand-Citrine
  • Insert Size (bp)
    3252
  • Promoter CMV
  • Tag / Fusion Protein
    • Lysosome-associated membrane protein 1 (LAMP1). (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Lyso-InPAkt was a gift from Jin Zhang (Addgene plasmid # 184053 ; http://n2t.net/addgene:184053 ; RRID:Addgene_184053)

  • For your References section:

    A Highly Sensitive Fluorescent Akt Biosensor Reveals Lysosome-Selective Regulation of Lipid Second Messengers and Kinase Activity. Chen M, Sun T, Zhong Y, Zhou X, Zhang J. ACS Cent Sci. 2021 Dec 22;7(12):2009-2020. doi: 10.1021/acscentsci.1c00919. Epub 2021 Dec 3. 10.1021/acscentsci.1c00919 PubMed 34963894
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