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Addgene

pEGFP_IRES_tdTomato
(Plasmid #184046)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184046 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3300
  • Total vector size (bp) 6261
  • Modifications to backbone
    F1 origin removed
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Enhanced Green Fluorescent Protein
  • Alt name
    EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGTCGCCGTCCAGCTCGACCAG
  • 3′ sequencing primer CATGGTCCTGCTGGAGTTCGTG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    tdTomato
  • Species
    Synthetic
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gatccgtacgGCCACCATGGTGAGCAAGG
  • 3′ sequencing primer GAGAATTAAGCTCTACTTGTACAGCTCGTCCATGC
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Internal Ribosome Entry Site
  • Alt name
    IRES
  • Species
    Human Poliovirus 1 Mahoney
  • Insert Size (bp)
    581
  • Promoter CMV

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTACAAGTAActcgagtactccggtattgcgg
  • 3′ sequencing primer ccatggtggccgtacggatcctatccaattcgc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP_IRES_tdTomato was a gift from Angela Pannier (Addgene plasmid # 184046 ; http://n2t.net/addgene:184046 ; RRID:Addgene_184046)
  • For your References section:

    Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells. Kozisek T, Samuelson L, Hamann A, Pannier AK. J Biol Eng. 2023 Dec 7;17(1):76. doi: 10.1186/s13036-023-00394-0. 10.1186/s13036-023-00394-0 PubMed 38062439