H2B-GFP-Aurora B KD
(Plasmid #184043)

• Purpose: Expresses H2B-GFP fused to Aurora B kinase dead
• Depositing Lab: Lienhard Schmitz
• Publication: Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 10.1098/rsob.230460. Epub 2024 May 29.

Backbone

• Vector backbone: H2B-EGFP
• Backbone size w/o insert (bp): 5111
• Total vector size (bp): 6078
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AURKB
• Alt name: Aurora B
• Species: H. sapiens (human)
• Insert Size (bp): 954
• Mutation: Changed Lysine 65 to Arginine
• GenBank ID: NM_001256834.3
• Promoter: CMV
• Tags / Fusion Proteins:
  • H2B (N terminal on backbone)
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GTCCTGCTGGAGTTCGTG

Resource Information

• Supplemental Documents:
  • H2B-EGFP-AurkB KD.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry