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Addgene

pCS2-mCherry-Rab11a (S25N)
(Plasmid #184036)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184036 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4091
  • Total vector size (bp) 5449
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab11
  • Species
    Oryctolagus cuniculus
  • Insert Size (bp)
    1359
  • Mutation
    Serine 25 changed to Asparagine
  • GenBank ID
    NM_001082251.2
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Stephen Doxsey Lab University of Massachusetts Medical School Program in Molecular Medicine Published in: Hehnly H, Doxsey S. Rab11 endosomes contribute to mitotic spindle organization and orientation. Developmental Cell. 28, 497-507. 2014. Hehnly and Doxsey, The Centrosome Regulates the Rab11- Dependent Recycling Endosome Pathway at Appendages of the Mother Centriole. Current Biology 20: 1944-50, 2012.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-mCherry-Rab11a (S25N) was a gift from Heidi Hehnly (Addgene plasmid # 184036 ; http://n2t.net/addgene:184036 ; RRID:Addgene_184036)
  • For your References section:

    Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo. Krishnan N, Swoger M, Rathbun LI, Fioramonti PJ, Freshour J, Bates M, Patteson AE, Hehnly H. Life Sci Alliance. 2022 Mar 18;5(7). pii: 5/7/e202201362. doi: 10.26508/lsa.202201362. Print 2022 Jul. 10.26508/lsa.202201362 PubMed 35304423