pCS2-mCherry-Rab11a (S25N)
(Plasmid
#184036)
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PurposeExpresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryos
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4091
- Total vector size (bp) 5449
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab11
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SpeciesOryctolagus cuniculus
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Insert Size (bp)1359
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MutationSerine 25 changed to Asparagine
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GenBank IDNM_001082251.2
- Promoter CMV IE94
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byStephen Doxsey Lab University of Massachusetts Medical School Program in Molecular Medicine Published in: Hehnly H, Doxsey S. Rab11 endosomes contribute to mitotic spindle organization and orientation. Developmental Cell. 28, 497-507. 2014. Hehnly and Doxsey, The Centrosome Regulates the Rab11- Dependent Recycling Endosome Pathway at Appendages of the Mother Centriole. Current Biology 20: 1944-50, 2012.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-mCherry-Rab11a (S25N) was a gift from Heidi Hehnly (Addgene plasmid # 184036 ; http://n2t.net/addgene:184036 ; RRID:Addgene_184036) -
For your References section:
Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo. Krishnan N, Swoger M, Rathbun LI, Fioramonti PJ, Freshour J, Bates M, Patteson AE, Hehnly H. Life Sci Alliance. 2022 Mar 18;5(7). pii: 5/7/e202201362. doi: 10.26508/lsa.202201362. Print 2022 Jul. 10.26508/lsa.202201362 PubMed 35304423