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Addgene

pCI-myc-USP10
(Plasmid #184034)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184034 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4006
  • Total vector size (bp) 7339
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    USP10
  • Species
    H. sapiens (human)
  • Entrez Gene
    USP10 (a.k.a. UBPO)
  • Promoter CMVf
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-myc-USP10 was a gift from Juan Bonifacino (Addgene plasmid # 184034 ; http://n2t.net/addgene:184034 ; RRID:Addgene_184034)

  • For your References section:

    The ubiquitin isopeptidase USP10 deubiquitinates LC3B to increase LC3B levels and autophagic activity. Jia R, Bonifacino JS. J Biol Chem. 2021 Jan-Jun;296:100405. doi: 10.1016/j.jbc.2021.100405. Epub 2021 Feb 10. 10.1016/j.jbc.2021.100405 PubMed 33577797
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