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Addgene

pcDNA flag-Msi1
(Plasmid #184029)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184029 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Msi1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Msi1 (a.k.a. Msi1h, Musahi1, m-Msi-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer T7
  • 3′ sequencing primer pcDNA3.1/BGH reverse priming site
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: BamHI (not destroyed), 3' Cloning Site: XbaI (not destroyed).

Please visit https://www.biorxiv.org/content/10.1101/2022.03.09.483688v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA flag-Msi1 was a gift from Peter Stoilov (Addgene plasmid # 184029 ; http://n2t.net/addgene:184029 ; RRID:Addgene_184029)

  • For your References section:

    The Musashi proteins direct post-transcriptional control of protein expression and alternate exon splicing in vertebrate photoreceptors. Matalkah F, Jeong B, Sheridan M, Horstick E, Ramamurthy V, Stoilov P. Commun Biol. 2022 Sep 24;5(1):1011. doi: 10.1038/s42003-022-03990-w. 10.1038/s42003-022-03990-w PubMed 36153373
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