pcDNA HA-Gnat1 WT-3UTR
(Plasmid
#184026)
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PurposeExpresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184026 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGnat1
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SpeciesM. musculus (mouse)
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Entrez GeneGnat1 (a.k.a. Gnat-1, Ird1, Ird2, Tralpha, irdc, irdr, transducin)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer pcDNA3.1/BGH reverse priming site (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDerived from MGC clone ID 6490797
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: XhoI (destroyed during cloning).
Please visit https://www.biorxiv.org/content/10.1101/2022.03.09.483688v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA HA-Gnat1 WT-3UTR was a gift from Peter Stoilov (Addgene plasmid # 184026 ; http://n2t.net/addgene:184026 ; RRID:Addgene_184026) -
For your References section:
The Musashi proteins direct post-transcriptional control of protein expression and alternate exon splicing in vertebrate photoreceptors. Matalkah F, Jeong B, Sheridan M, Horstick E, Ramamurthy V, Stoilov P. Commun Biol. 2022 Sep 24;5(1):1011. doi: 10.1038/s42003-022-03990-w. 10.1038/s42003-022-03990-w PubMed 36153373