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Addgene

pcDNA-Prom1 D+3
(Plasmid #184022)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184022 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prom1 SV8(-Ex19a) D+3
  • Species
    M. musculus (mouse)
  • Mutation
    Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 721 to 735
  • Entrez Gene
    Prom1 (a.k.a. 4932416E19Rik, AC133, CD133, Prom, Prom-1, Proml1)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from MGC clone ID: 4502359

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: XbaI (not destroyed). Please visit https://doi.org/10.1101/2022.03.04.482347 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Prom1 D+3 was a gift from Peter Stoilov (Addgene plasmid # 184022 ; http://n2t.net/addgene:184022 ; RRID:Addgene_184022)

  • For your References section:

    The mAB 13A4 monoclonal antibody to the mouse PROM1 protein recognizes a structural epitope. Matalkah F, Rhodes S, Ramamurthy V, Stoilov P. PLoS One. 2022 Oct 10;17(10):e0274958. doi: 10.1371/journal.pone.0274958. eCollection 2022. 10.1371/journal.pone.0274958 PubMed 36215230
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