AAV2/9-CW3SL-mCherry-DrTrkB
(Plasmid
#184002)
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PurposeAAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2/9-CW3SL
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeDrTrkB
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SpeciesH. sapiens (human)
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Entrez GeneNTRK2 (a.k.a. DEE58, EIEE58, GP145-TrkB, OBHD, TRKB, trk-B)
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Tag
/ Fusion Protein
- Ig kappa secretory signal, mCherry, Deinococcus radiodurans bacterial phytochrome (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV2/9-CW3SL-mCherry-DrTrkB was a gift from Dmitry Gerashchenko & Vladislav Verkhusha (Addgene plasmid # 184002 ; http://n2t.net/addgene:184002 ; RRID:Addgene_184002) -
For your References section:
A general approach for engineering RTKs optically controlled with far-red light. Leopold AV, Thankachan S, Yang C, Gerashchenko D, Verkhusha VV. Nat Methods. 2022 Jul;19(7):871-880. doi: 10.1038/s41592-022-01517-z. Epub 2022 Jun 9. 10.1038/s41592-022-01517-z PubMed 35681062