eDrcKIT
(Plasmid
#184001)
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PurposeOptically controlled eDrcKIT expression in mammalian cells
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeDrcKIT
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SpeciesH. sapiens (human)
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Entrez GeneKIT (a.k.a. C-Kit, CD117, MASTC, PBT, SCFR)
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Tag
/ Fusion Protein
- Ig kappa secretory signal, mCherry, Deinococcus radiodurans bacterial phytochrome (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
eDrcKIT was a gift from Dmitry Gerashchenko & Vladislav Verkhusha (Addgene plasmid # 184001 ; http://n2t.net/addgene:184001 ; RRID:Addgene_184001) -
For your References section:
A general approach for engineering RTKs optically controlled with far-red light. Leopold AV, Thankachan S, Yang C, Gerashchenko D, Verkhusha VV. Nat Methods. 2022 Jul;19(7):871-880. doi: 10.1038/s41592-022-01517-z. Epub 2022 Jun 9. 10.1038/s41592-022-01517-z PubMed 35681062
