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Addgene

eDrIR1
(Plasmid #183998)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183998 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eDrIR1
  • Species
    H. sapiens (human)
  • Entrez Gene
    INSR (a.k.a. CD220, HHF5)
  • Tag / Fusion Protein
    • Ig kappa secretory signal, mCherry, Deinococcus radiodurans bacterial phytochrome (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    eDrIR1 was a gift from Dmitry Gerashchenko & Vladislav Verkhusha (Addgene plasmid # 183998 ; http://n2t.net/addgene:183998 ; RRID:Addgene_183998)

  • For your References section:

    A general approach for engineering RTKs optically controlled with far-red light. Leopold AV, Thankachan S, Yang C, Gerashchenko D, Verkhusha VV. Nat Methods. 2022 Jul;19(7):871-880. doi: 10.1038/s41592-022-01517-z. Epub 2022 Jun 9. 10.1038/s41592-022-01517-z PubMed 35681062
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