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OA-986F (AAEL003877-dCas9-VPR)
(Plasmid #183993)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183993 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Plasmid #100581
  • Backbone size w/o insert (bp) 5246
  • Total vector size (bp) 14840
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAEL003877-dCAS9-VPR
  • Species
    Aedes aegypti
  • Insert Size (bp)
    7134
  • Promoter AAEL003887
  • Tag / Fusion Protein
    • dCas9-VPR

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TATCTTTACATGTAGCTTGTGCATTGAA
  • 3′ sequencing primer CCGGCCGTTAACTCGAATCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OA-986F (AAEL003877-dCas9-VPR) was a gift from Omar Akbari (Addgene plasmid # 183993 ; http://n2t.net/addgene:183993 ; RRID:Addgene_183993)

  • For your References section:

    CRISPR mediated transactivation in the human disease vector Aedes aegypti. Bui M, Dalla Benetta E, Dong Y, Zhao Y, Yang T, Li M, Antoshechkin IA, Buchman A, Bottino-Rojas V, James AA, Perry MW, Dimopoulos G, Akbari OS. PLoS Pathog. 2023 Jan 19;19(1):e1010842. doi: 10.1371/journal.ppat.1010842. eCollection 2023 Jan. 10.1371/journal.ppat.1010842 PubMed 36656895
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