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Addgene

H2B-HT-emiRFP703
(Plasmid #183985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2226
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Tag / Fusion Protein
    • HaloTag, emiRFP703 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2B-HT-emiRFP703 was a gift from Pablo Rivera-Fuentes (Addgene plasmid # 183985 ; http://n2t.net/addgene:183985 ; RRID:Addgene_183985)

  • For your References section:

    A locally activatable sensor for robust quantification of organellar glutathione. Emmert S, Quargnali G, Thallmair S, Rivera-Fuentes P. Nat Chem. 2023 Oct;15(10):1415-1421. doi: 10.1038/s41557-023-01249-3. Epub 2023 Jun 15. 10.1038/s41557-023-01249-3 PubMed 37322101
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