TDRD3:ABC006-C02:C220502
(Plasmid
#183982)
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PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepCW-FAB2HIS-LIC-(vector is edited from pCWori)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTDRD3-anti-TDRD3 FAB
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Alt name3PNW-chain A and B
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Alt nameBC030514
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SpeciesH. sapiens (human)
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Insert Size (bp)1558
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Entrez GeneTDRD3 (a.k.a. RP11-459E2.1, FLJ21007)
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Tags
/ Fusion Proteins
- anti-TDRD3 FAB HC: N-terminal STII leader peptide; anti-TDRD3 FAB LC: N-terminal pelB leader peptide (N terminal on insert)
- anti-TDRD3 FAB HC: C-terminal HIS tag; anti-TDRD3 FAB LC: C-terminal flag tag (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SGC Clone Sample ID: TDRD3:ABC006-C02:C220502. SGC link: http://www.thesgc.org/structures/3PNW/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TDRD3:ABC006-C02:C220502 was a gift from Cheryl Arrowsmith (Addgene plasmid # 183982 ; http://n2t.net/addgene:183982 ; RRID:Addgene_183982)