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Addgene

TDRD3:ABC006-C02:C220502
(Plasmid #183982)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 183982 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pCW-FAB2HIS-LIC-(vector is edited from pCWori)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TDRD3-anti-TDRD3 FAB
  • Alt name
    3PNW-chain A and B
  • Alt name
    BC030514
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1558
  • Entrez Gene
    TDRD3 (a.k.a. RP11-459E2.1, FLJ21007)
  • Tags / Fusion Proteins
    • anti-TDRD3 FAB HC: N-terminal STII leader peptide; anti-TDRD3 FAB LC: N-terminal pelB leader peptide (N terminal on insert)
    • anti-TDRD3 FAB HC: C-terminal HIS tag; anti-TDRD3 FAB LC: C-terminal flag tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SGC Clone Sample ID: TDRD3:ABC006-C02:C220502. SGC link: http://www.thesgc.org/structures/3PNW/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TDRD3:ABC006-C02:C220502 was a gift from Cheryl Arrowsmith (Addgene plasmid # 183982 ; http://n2t.net/addgene:183982 ; RRID:Addgene_183982)