TDRD3:JMC006-H10:C42283
(Plasmid
#183981)
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PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerPlasmid # 26096
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTDRD3 in Complex with anti-TDRD3 FAB
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Alt name3PNW-chain C
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Alt namegi|13540575
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SpeciesH. sapiens (human)
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Insert Size (bp)228
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Entrez GeneTDRD3 (a.k.a. RP11-459E2.1, FLJ21007)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SGC Clone Sample ID: TDRD3:JMC006-H10:C42283. SGC link: http://www.thesgc.org/structures/3PNW/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TDRD3:JMC006-H10:C42283 was a gift from Cheryl Arrowsmith (Addgene plasmid # 183981 ; http://n2t.net/addgene:183981 ; RRID:Addgene_183981)