RFXANK:JMC029-A02:C213521
(Plasmid
#183980)
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PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerPlasmid # 26096
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRFXANK
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Alt name3V30
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Alt nameBC114563/gi|19924154
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SpeciesH. sapiens (human)
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Insert Size (bp)513
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Entrez GeneRFXANK (a.k.a. ANKRA1, BLS, F14150_1, RFX-B)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-TEV (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
SGC Clone Sample ID: 'RFXANK:JMC029-A02:C213521. SGC link: http://www.thesgc.org/structures/3V30/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RFXANK:JMC029-A02:C213521 was a gift from Cheryl Arrowsmith (Addgene plasmid # 183980 ; http://n2t.net/addgene:183980 ; RRID:Addgene_183980)