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LIPS:SDC101a-A08:C34411
(Plasmid #183978)

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Item Catalog # Description Quantity Price (USD)
Plasmid 183978 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pAB- LIC C
  • Backbone manufacturer
    Plasmid #62288
  • Vector type
    Baculovirus Expresssion

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LIPS
  • Alt name
    2OXE
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1356
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • N-terminal Honeybee melittin signal:MKFLVNVALVFMVVYISYIYAAA (N terminal on insert)
    • C-terminal 8xHis tag:EFVEHHHHHHHH (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer phF:AGACGCACAAACTAATATCACAAACTGGA
  • 3′ sequencing primer mR:CGTGTCGGGTTTAACATTACGGAT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SGC Clone Sample ID: LIPS:SDC101a-A08:C34411. PDB link: https://www.rcsb.org/structure/2OXE

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LIPS:SDC101a-A08:C34411 was a gift from Cheryl Arrowsmith (Addgene plasmid # 183978 ; http://n2t.net/addgene:183978 ; RRID:Addgene_183978)
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