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Addgene

DCAF1:PBC020-D05:C246337
(Plasmid #183974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFBOH-MHL (Addgene #62304)
  • Vector type
    Baculovirus Expresssion

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DCAF1:PBC020-D05:C246337
  • Alt name
    DCAF1, DCAF1_S01
  • Alt name
    PDB IDs: 7SSE, 8F8E
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    942
  • Entrez Gene
    DCAF1 (a.k.a. RIP, VPRBP)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • MHHHHHHSSGRENLYFQG (N terminal on backbone)
    • 6xHis-TEV (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DCAF1:PBC020-D05:C246337 was a gift from Cheryl Arrowsmith (Addgene plasmid # 183974 ; http://n2t.net/addgene:183974 ; RRID:Addgene_183974)
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