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Addgene

T444T-GFP(NCO)
(Plasmid #183972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183972 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    T444T
  • Backbone size w/o insert (bp) 2132
  • Total vector size (bp) 2846
  • Vector type
    Worm Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    HT115
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP (non-codon-optimized)
  • Species
    Synthetic
  • Insert Size (bp)
    734

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GFP insert was synthesized by Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T444T-GFP(NCO) was a gift from David Matus (Addgene plasmid # 183972 ; http://n2t.net/addgene:183972 ; RRID:Addgene_183972)

  • For your References section:

    A laboratory module that explores RNA interference and codon optimization through fluorescence microscopy using Caenorhabditis elegans. Palmisano NJ, Azmi MA, Medwig-Kinney TN, Moore FEQ, Rahman R, Zhang W, Adikes RC, Matus DQ. CourseSource, 2022 10.24918/cs.2022.15
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