pB PUb-integrase
(Plasmid #183966)

• Purpose: piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
• Depositing Lab: Eric Marois
• Publication: Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi: 10.1038/s42003-023-05030-7.

Backbone

• Vector backbone: piggyBac GGC compatible vector (Addgene # 173496)
• Vector type: piggyBac insect transgenesis destination vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PhiC31 integrase
• Species: Streptomyces
• Promoter: Aedes aegypti Polyubiquitin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (Golden Gate Cloning) (destroyed during cloning)
• 3′ cloning site: BsaI (Golden Gate Cloning) (destroyed during cloning)
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • 1315 ppBnoattnoloxPUb-phiC31integrase.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be used a helper plasmid to express integrase during micro-injection and mediate integration of attB-containing plasmids into genomic attP sites.

Please visit https://doi.org/10.1101/2022.03.11.483912 for bioRxiv preprint.