pPolI_SARS-CoV-2_NLuc
(Plasmid #183953)

• Purpose: Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
• Depositing Lab: AJ te Velthuis
• Publication: Vial et al bioRxiv 2021.06.28.450211

Backbone

• Vector backbone: pMK-RQ
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2278
• Total vector size (bp): 4830
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 minigenome with Nano luciferase
• Species: SARS coronavirus 2
• Insert Size (bp): 2552
• GenBank ID: NC_045512.2
• Entrez Gene: N (a.k.a. GU280_gp10)
• Entrez Gene: ORF10 (a.k.a. GU280_gp11)
• Promoter: Mammalian Pol I

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GTGAGCGCGACGTAATAC
• 3′ sequencing primer: GCTGGCACGACAGGTTTC

Resource Information

• Supplemental Documents:
  • 20ACX4OP_2907589_pPolI_SARS2_NLuc.gb
• A portion of this plasmid was derived from a plasmid made by: Invitrogen/GeneArt: The synthetic gene pPolI_SARS2_NLuc was assembled from synthetic oligonucleotides and/or PCR products by Invitrogen/GeneArt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.06.28.450211v2 for bioRxiv preprint.