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pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1
(Plasmid #183951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIX_403 (Addgene 41395)
  • Backbone manufacturer
    Root Lab, Addgene
  • Backbone size w/o insert (bp) 9396
  • Total vector size (bp) 9152
  • Modifications to backbone
    LR insertion of place-holder gene, followed by restriction cloning and Gibson assembly to isolate APOBEC1 and insert Capture1.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APOBEC1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    687
  • Entrez Gene
    Apobec1 (a.k.a. REPR, apobec-1)
  • Promoter TRE promoter, Tet ON

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1 was a gift from Eugene Yeo (Addgene plasmid # 183951 ; http://n2t.net/addgene:183951 ; RRID:Addgene_183951)

  • For your References section:

    Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes. Brannan KW, Chaim IA, Marina RJ, Yee BA, Kofman ER, Lorenz DA, Jagannatha P, Dong KD, Madrigal AA, Underwood JG, Yeo GW. Nat Methods. 2021 May;18(5):507-519. doi: 10.1038/s41592-021-01128-0. Epub 2021 May 7. 10.1038/s41592-021-01128-0 PubMed 33963355
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