p416-GPD-DIPP3A-HA
(Plasmid
#183948)
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PurposeExpresses human HA-tagged DIPP3A from yeast GPD promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183948 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep416
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDIPP3A
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Alt nameNUDT10
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SpeciesH. sapiens (human)
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MutationWT
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Entrez GeneNUDT10 (a.k.a. APS2, DIPP3a, hDIPP3alpha)
- Promoter GPD
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p416-GPD-DIPP3A-HA was a gift from Michael Downey (Addgene plasmid # 183948 ; http://n2t.net/addgene:183948 ; RRID:Addgene_183948)