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Addgene

pKSB-AeU6-1gRNA
(Plasmid #183912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKSB- (Addgene #62540)
  • Backbone size w/o insert (bp) 2961

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA scaffold for protospacer cloning into BbsI sites
  • Promoter Aedes aegypti U6 promoter (AAEL017702)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (Golden Gate Cloning) (destroyed during cloning)
  • 3′ cloning site BsaI (Golden Gate Cloning) (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

to express a gRNA in Aedes aegypti. Module can be incorporated in a GoldenGate assembly using the BsaI sites. Please visit https://doi.org/10.1101/2022.03.11.483912 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKSB-AeU6-1gRNA was a gift from Eric Marois (Addgene plasmid # 183912 ; http://n2t.net/addgene:183912 ; RRID:Addgene_183912)

  • For your References section:

    Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control. Lutrat C, Burckbuchler M, Olmo RP, Beugnon R, Fontaine A, Akbari OS, Argiles-Herrero R, Baldet T, Bouyer J, Marois E. Commun Biol. 2023 Jun 16;6(1):646. doi: 10.1038/s42003-023-05030-7. 10.1038/s42003-023-05030-7 PubMed 37328568
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