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Addgene

pX4
(Plasmid #183903)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183903 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR R4 ATCC LacZGCTT (Addgene #173668)
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 6785
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Alt name
    3xP3-GFP-DmTub56Dterminator cassette flanked by Ae. aegypti genomic sequences
  • Species
    Synthetic
  • Promoter Aedes aegypti PUb

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (Golden Gate Cloning) (destroyed during cloning)
  • 3′ cloning site BsaI (Golden Gate Cloning) (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Donor construct designed to insert GFP near the m/M locus of Aedes aegypti using the CRISPR/Cas9 system.

Please visit https://doi.org/10.1101/2022.03.11.483912 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX4 was a gift from Eric Marois (Addgene plasmid # 183903 ; http://n2t.net/addgene:183903 ; RRID:Addgene_183903)

  • For your References section:

    Combining two genetic sexing strains allows sorting of non-transgenic males for Aedes genetic control. Lutrat C, Burckbuchler M, Olmo RP, Beugnon R, Fontaine A, Akbari OS, Argiles-Herrero R, Baldet T, Bouyer J, Marois E. Commun Biol. 2023 Jun 16;6(1):646. doi: 10.1038/s42003-023-05030-7. 10.1038/s42003-023-05030-7 PubMed 37328568
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