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Purpose(Empty Backbone) Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLIX_403 (Addgene 41395)
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Backbone manufacturerRoot Lab, Addgene
- Backbone size (bp) 9396
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Modifications to backbonepLIX403 inducible lentiviral expression vector adapted from pLIX_403 (deposited by D. Root; Addgene plasmid no. 41395) to contain TRE-gateway-APOBEC-Capture1-HA-P2A-mRuby with PGK-puro-T2A-rtTA downstream of mRuby.
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Vector typeMammalian Expression, Lentiviral ; Gateway Destination vector, Doxycycline inducible
- Promoter TRE promoter, Tet ON
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- Capture1_APOBEC_HA_P2A_mRuby (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer LNCX
- 3′ sequencing primer O.PGK1b-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIX403_Capture1_APOBEC_HA_P2A_mRuby was a gift from Eugene Yeo (Addgene plasmid # 183901 ; http://n2t.net/addgene:183901 ; RRID:Addgene_183901) -
For your References section:
Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes. Brannan KW, Chaim IA, Marina RJ, Yee BA, Kofman ER, Lorenz DA, Jagannatha P, Dong KD, Madrigal AA, Underwood JG, Yeo GW. Nat Methods. 2021 May;18(5):507-519. doi: 10.1038/s41592-021-01128-0. Epub 2021 May 7. 10.1038/s41592-021-01128-0 PubMed 33963355
Map uploaded by the depositor.
