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pLenti-EML4-ALK variant 3a L1196M/G1202R
(Plasmid #183831)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 183831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti
  • Backbone size w/o insert (bp) 7877
  • Total vector size (bp) 10235
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ALK Receptor Tyrosine Kinase
  • Alt name
    ALK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1691
  • Mutation
    L1196M/G1202R
  • GenBank ID
    NM_004304
  • Entrez Gene
    ALK (a.k.a. ALK1, CD246, NBLST3)
  • Promoter CMV
  • Tag / Fusion Protein
    • The fusion protein of EML4 exon 1-6a and ALK exon 20-29

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    EMAP Like 4
  • Alt name
    EML4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    667
  • GenBank ID
    NM_019063.5
  • Entrez Gene
    EML4 (a.k.a. C2orf2, ELP120, EMAP-4, EMAPL4, ROPP120)
  • Promoter CMV
  • Tag / Fusion Protein
    • The fusion protein of EML4 exon 1-6a and ALK exon 20-29

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-EML4-ALK variant 3a L1196M/G1202R was a gift from Aaron Hata (Addgene plasmid # 183831 ; http://n2t.net/addgene:183831 ; RRID:Addgene_183831)

  • For your References section:

    Sequential ALK Inhibitors Can Select for Lorlatinib-Resistant Compound ALK Mutations in ALK-Positive Lung Cancer. Yoda S, Lin JJ, Lawrence MS, Burke BJ, Friboulet L, Langenbucher A, Dardaei L, Prutisto-Chang K, Dagogo-Jack I, Timofeevski S, Hubbeling H, Gainor JF, Ferris LA, Riley AK, Kattermann KE, Timonina D, Heist RS, Iafrate AJ, Benes CH, Lennerz JK, Mino-Kenudson M, Engelman JA, Johnson TW, Hata AN, Shaw AT. Cancer Discov. 2018 Jun;8(6):714-729. doi: 10.1158/2159-8290.CD-17-1256. Epub 2018 Apr 12. 10.1158/2159-8290.CD-17-1256 PubMed 29650534
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